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transam ® nf-kb activation assay kit  (Active Motif)

 
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    Active Motif transam ® nf-kb activation assay kit
    Transam ® Nf Kb Activation Assay Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transam ® nf-kb activation assay kit/product/Active Motif
    Average 90 stars, based on 1 article reviews
    transam ® nf-kb activation assay kit - by Bioz Stars, 2026-04
    90/100 stars

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    EZH2-mediated H3K27me3 signature contributes to NF- κ B p65-dependent inflammatory changes. A , C Effect of NADPH oxidase inhibitor apocynin (100 µmol/l) and GSK126 (5 µmol/l) on high glucose-induced increase of NF-κB p65 binding activity (n = 3–6/group). B , D RT-qPCR showing gene expression of inflammatory markers (n = 6/group) in HAECs exposed to high glucose alone in the presence of apocynin (100 µmol/l), GSK126 (5 µmol/l), or vehicle alone. E Representative images and relative quantification of monocyte adhesion to HAEC exposed to high glucose in the presence and in the absence of TNFa (5 mmol/l) and treated with GSK126 (5 µmol/l) or vehicle alone (n = 3/group). Scale bar = 100 μm. IL-6 interleukin-6, TNFα tumor necrosis factor α, MCP-1 monocyte chemoattractant factor-1, ICAM-1 intercellular adhesion molecule 1, VCAM-1 vascular cell adhesion molecule 1

    Journal: Cardiovascular Diabetology

    Article Title: Repressive H3K27me3 drives hyperglycemia-induced oxidative and inflammatory transcriptional programs in human endothelium

    doi: 10.1186/s12933-024-02196-0

    Figure Lengend Snippet: EZH2-mediated H3K27me3 signature contributes to NF- κ B p65-dependent inflammatory changes. A , C Effect of NADPH oxidase inhibitor apocynin (100 µmol/l) and GSK126 (5 µmol/l) on high glucose-induced increase of NF-κB p65 binding activity (n = 3–6/group). B , D RT-qPCR showing gene expression of inflammatory markers (n = 6/group) in HAECs exposed to high glucose alone in the presence of apocynin (100 µmol/l), GSK126 (5 µmol/l), or vehicle alone. E Representative images and relative quantification of monocyte adhesion to HAEC exposed to high glucose in the presence and in the absence of TNFa (5 mmol/l) and treated with GSK126 (5 µmol/l) or vehicle alone (n = 3/group). Scale bar = 100 μm. IL-6 interleukin-6, TNFα tumor necrosis factor α, MCP-1 monocyte chemoattractant factor-1, ICAM-1 intercellular adhesion molecule 1, VCAM-1 vascular cell adhesion molecule 1

    Article Snippet: NF-κB p65 binding activity in HAEC and D-HAEC was measured by the TransAMTM NF-κB p65 activation protein assay kit (Active Motif; Carlsbad, CA, USA), following the manufacturer’s instructions.

    Techniques: Binding Assay, Activity Assay, Quantitative RT-PCR, Gene Expression, Quantitative Proteomics

    Targeting EZH2-H3K27me3 epigenetic signature rescues abnormal phenotypes in endothelial cells isolated from patients with diabetes. A Representative confocal microscopy images of H3K27me3 (green), EZH2 (red), and EZH2/H3K27me3 colocalization (yellow) and densitometric quantification of fluorescence intensity. Cell nuclei are stained with Hoechst (blue). Scale bar = 2 μm. n = 6/group. B and C RT-qPCR arrays showing gene expression of SOD1 , SOD2 , JunD , and C NOX4 , respectively (n = 3/group). D electron spin resonance (ESR) spectroscopy analysis of O 2 − production (n = 3/group). E NF-κB p65 binding activity (n = 6/group), and F gene expression of IL-6 and MCP-1 (n = 3/group). IL-6, interleukin-6; MCP-1, monocyte chemoattractant factor-1

    Journal: Cardiovascular Diabetology

    Article Title: Repressive H3K27me3 drives hyperglycemia-induced oxidative and inflammatory transcriptional programs in human endothelium

    doi: 10.1186/s12933-024-02196-0

    Figure Lengend Snippet: Targeting EZH2-H3K27me3 epigenetic signature rescues abnormal phenotypes in endothelial cells isolated from patients with diabetes. A Representative confocal microscopy images of H3K27me3 (green), EZH2 (red), and EZH2/H3K27me3 colocalization (yellow) and densitometric quantification of fluorescence intensity. Cell nuclei are stained with Hoechst (blue). Scale bar = 2 μm. n = 6/group. B and C RT-qPCR arrays showing gene expression of SOD1 , SOD2 , JunD , and C NOX4 , respectively (n = 3/group). D electron spin resonance (ESR) spectroscopy analysis of O 2 − production (n = 3/group). E NF-κB p65 binding activity (n = 6/group), and F gene expression of IL-6 and MCP-1 (n = 3/group). IL-6, interleukin-6; MCP-1, monocyte chemoattractant factor-1

    Article Snippet: NF-κB p65 binding activity in HAEC and D-HAEC was measured by the TransAMTM NF-κB p65 activation protein assay kit (Active Motif; Carlsbad, CA, USA), following the manufacturer’s instructions.

    Techniques: Isolation, Confocal Microscopy, Fluorescence, Staining, Quantitative RT-PCR, Gene Expression, Electron Paramagnetic Resonance, Spectroscopy, Binding Assay, Activity Assay